@article{oai:jissen.repo.nii.ac.jp:00001007, author = {荒木, 紀美 and 中田, 陽子 and 菱田, 桂菜 and 池和田, 恵 and 谷亀, 久美子 and 小林, 幹彦 and アラキ, モトミ and ナカダ, ヨウコ and ヒシダ, ケイナ and イケワダ, メグミ and ヤガメ, クミコ and コバヤシ, ミキヒコ and Araki, Motomi and Nakada, Yoko and Hishida, Keina and Ikewada, Megumi and Yagame, Kumiko and Kabayashi, Mikihiko}, journal = {実践女子大学生活科学部紀要}, month = {Mar}, note = {P(論文), Carbohydrate components and their related carbohydrate activities in a homogenized preparation of the fruit body of king oyster mushroom (eryngii), Pleurotus eryngii, were studied in detail. The low-molecular weight fractions contained a significant amount of trehalose compared with other constituents of mono-saccharides, and its concentration was 18.7% of the dry weight. Polysaccharides in the high-molecular weight fraction were separated into four fractions, A~D, which were analyzed for the yields and sugar components. The polysaccharide fractions were also analyzed by the ion-exchange and gel-filtration column chromatography, and enzymatic hydrolysis. The results suggested that typical polysaccharide components in the fruit body of eryngii might be β-glucan and galactan, corresponding to the known results obtained by the liquid-cultured eryngii. A crude enzyme solution was prepared from the fruit body of eryngii, and various carbohydrase activities were measured using 27 substrates at pH 5.2, 6.5 and 7.8. A higher enzyme activity was detected for the substrates of laminarin and trehalose. Occurrence of trehalase activity correlated with the high concentration of trehalose in the fruit body of eringii.}, pages = {1--8}, title = {エリンギ(Pleurotus eryngii)の子実態における糖質および糖質分解酵素の特徴}, volume = {46}, year = {2009} }